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Image Search Results
Journal: Clinical and Experimental Immunology
Article Title: Laryngeal transplantation in minipigs: early immunological outcomes
doi: 10.1111/j.1365-2249.2011.04531.x
Figure Lengend Snippet: Antibodies, isotypes and combinations used in multiple colour immunofluorescence studies of the laryngeal mucosa. Five combinations, each of three antibodies, were used
Article Snippet: Percentage areas of positive pixel staining were calculated for each fluorochrome and fluorochrome combination. table ft1 table-wrap mode="anchored" t5 caption a7 Primary antibody † to Clone Isotype Secondary antibody ‡ False colour Combination Myeloid cells CD172 742215 IgG2B IgG2b-FITC a Green 1 CD163 2A/10/11 IgG1 IgG1 - TRITC a Red 1 MHC-II MSA3 EPICS IgG2A IgG2a-AF633 b Blue 1 and 2 CD14 MIL2 IgG2B IgG2b-FITC a Green 2 CD16 c G7 IgG1 IgG1-TRITC a Red 2 Lymphoid cells CD45RC MIL15 IgM IgM-FITC a Green 3 and 4 CD25 K231.3B2 IgG1 IgG1-TRITC a Red 3 and 4 CD4 MIL17 H-M IgG2B IgG2b-AF633 b Blue 3 CD8 MIL12 IgG2A IgG2a-AF633 b Blue 4 and 5
Techniques: Immunofluorescence
Journal: Journal of Virology
Article Title: WDR5 Facilitates Human Cytomegalovirus Replication by Promoting Capsid Nuclear Egress
doi: 10.1128/JVI.00207-18
Figure Lengend Snippet: Antibodies used in this study
Article Snippet: Membranes were sequentially probed with primary antibodies and appropriate peroxidase-conjugated secondary antibodies , developed using SuperSignal West Femto chemiluminescent substrate (catalog number 34095; Life Technologies), detected using a FluorChem HD2 system (Alpha Innotech), and quantitated by densitometry using ImageJ software (National Institutes of Health). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Antibody a (clone) Species/isotype Source/catalog number Primary antibodies Anti-WDR5 Rabbit polyclonal Millipore/07-706 Antiactin (clone 6G3) Mouse monoclonal/IgG1 Sungene Biotech/KM9001 Anti-ubiquitin B Rabbit polyclonal Abclonal/A0162 Anti-IE1 (clone p63-27) Mouse monoclonal/IgG2a Gift from William J. Britt, University of Alabama, USA Anti-IE1/2 (clone CH160) Mouse monoclonal/IgG1 Virusys/P1215 Anti-UL44 (clone CH13) Mouse monoclonal/IgG1 Virusys/P1202-1 Anti-gB (clone CH28) Mouse monoclonal/IgG1 Virusys/P1201 Anti-pUL53 (clone 7H8) Mouse monoclonal/IgG1 Gift from Stipan Jonjic, University of Rijeka, Rijeka, Croatia Anti-MCP (clone 28.4) Mouse monoclonal/IgG2a Gift from William J. Britt, University of Alabama, USA Secondary antibodies TRITC-anti-mouse IgG2a Goat
Techniques: